GraphPad Prism 5.0

Features of GraphPad Prism

Curve fitting

  • Marquardt-Levenberg nonlinear regression.
  • Fit data to a built-in classic equation, choose from an equation library, or enter your own.
  • Built-in equations include kinetic equations (exponential decay, growth, association), equilibrium mass action equations (binding isotherm, sigmoidal dose-response, competitive binding including 2 site), polynomial up to 4th order, Gaussian distribution, power series and sine.
  • Enter multiple-line user-defined equations, including If-Then clauses. Define different equations for different data sets (columns).
  • Generate a family of theoretical curves.
  • Create equation libraries to distribute to others.
  • Fit data to two equations and compare fits with an F test. Automatically graph the best fit.
  • Fit several data sets at once, sharing selected parameters between the data sets.
  • Compare two curves by comparing the sum of squares when selected parameters are shared between the data sets with the individual sum of squares.
  • Compare models (and data sets) using the extra sum-of-squares F test or Akaike's Information Criterion (AIC).

Data management

  • Enter Y data as raw replicates or mean, SD/SEM and N.
  • Automatic updating of analyses and graphs as data change.
  • Enter up to 104 related data sets on one table to be analyzed and plotted together. Up to 52 replicates and any number of points per data set
  • Accurately accounts for missing values.
  • Exclude specified points from analyses and graphs (they show on the table in blue italics, but are ignored by the analyses and graphs).
  • Transfer data to and from Excel and other applications with the clipboard, or import and export text (ASCII) files.
  • Import text files and retain the link. Double click on the Prism data to edit the text file.
  • Choose rows and columns to import.
  • Filter row. Choose to start importing after a condition is met, and to stop importing when another condition is met.
  • Filter columns. Choose a range of columns to import.
  • Decimate. Skip several rows after every row that is imported.
  • Unstack indexed data.
  • Transpose rows and columns.
  • Import free-form notes and structured info constants along with data.
  • Copy and paste data and results between Prism and Excel.
  • Paste a link from Excel into Prism. Double-click on the Prism data to return to Excel (Windows only).
  • Embed an entire spreadsheet file within a Prism file (Windows only).
  • Import Excel files directly (Windows only).
  • Normalise, sort or transpose data.
  • Subtract (or divide by) a baseline or compute relative changes.
  • Consolidate related data sets by calculating means or totals.
  • Transform data with a full range of mathematical functions, including user-defined transforms.
  • Smooth curves, or convert to their derivative or integral.

Statistics

  • Calculate slope and intercept with confidence intervals.
  • Force regression lines through a specified point.
  • Fit to replicate Y values or mean Y.
  • Test for departure from linearity with a runs test.
  • Calculate and graph residuals.
  • Compare slopes and intercepts of two or more regression lines.
  • Determine new points along the standard curve.
  • Pearson or Spearman (nonparametric) correlation.
  • Table of XY coordinates.
  • Calculate min, max, quartiles, mean, SD, SEM, CI, CV, Geometric mean with Confidence Intervals.
  • Specify desired level of confidence.
  • Frequency distributions (bin to histogram), including cumulative histograms.
  • Kolgoromov-Smirnov normality test.
  • One sample t test or Wilcoxon test to compare the column mean (or median) with a theoretical value.
  • Skewness and Kurtosis.
  • Kaplan-Meier survival analysis. Compare curves with the log-rank test (including test for trend).
  • Paired or unpaired t tests.
  • Mann-Whitney or Wilcoxon tests.
  • Ordinary or repeated measures one-way ANOVA with Tukey, Newman-Keuls, Dunnett or Bonferroni post tests, or the post-test for trend.
  • Kruskal-Wallis or Friedman nonparametric one-way ANOVA with Dunn's post test.
  • Fisher's exact test or the chi-square test. Calculate the relative risk and odds ratio with confidence intervals.
  • Two-way ANOVA, even with missing values with some post tests.
  • Repeated measures two-way ANOVA with some post tests.
  • Bland-Altman plots.
  • Receiver operator characteristic (ROC) curves.
  • Deming regression (type ll linear regression).

Graphing

  • Linear, log10, or log2 scale, ascending or descending numbering.
  • Segment axes with up to two breaks (gaps) per axis.
  • Select orientation of tick marks and adjust major and minor intervals. Choose tick length.
  • Label ticks using decimal, scientific, 10^X, or notation.
  • Place custom ticks and labels anywhere on an axis.
  • Offset X and Y axes.
  • Add a right-hand Y axis.
  • Choose number of decimal points for axis numbering.
  • Precede axis numbers with a prefix (i.e. "$") and follow with a suffix (i.e. "%")
  • Numbering under X axis can be at any angle.
  • Move numbering and axis title closer to, or further from, the axis.
  • XY graphs, bar graphs (vertical or horizontal), box-and-whisker plots, and scatter plots.
  • Plot hundreds data sets on a single graph. Add or remove data sets.
  • Create graphs with no frame, a plain frame, a frame with ticks, or a frame with a grid.
  • Adjust size and shape of graph by specifying dimensions or dragging with the mouse.
  • 14 different symbols in 9 sizes.
  • 5 line patterns (dotted, dashed, etc.) in 7 line thicknesses and 16 colors.
  • Connect points with a line, staircase, or spline curve.
  • Start point-to-point lines at the origin or the first data point.
  • Change origin position: lower left, automatically selected, or custom.
  • Built-in drawing tools--lines, arrows, boxes, circles, ovals, and arcs.
  • Automatic calculation of error bars from replicate values.
  • Plot mean and SD, mean and SEM, mean and 95% CI, mean and range, median and range, or median and interquartile range.
  • Error bars can be above or below the point, or both, and with caps or without.
  • Specify error bar thickness.
  • Option to suppress error bars.
  • Automatic legends that are customisable.
  • Easily access Greek letters, mathematical symbols, or international characters.
  • Rotate text any angle.
  • Add underlining, bold, super and subscripts, or change point size easily from the tool bar.
  • Move graph and axis titles without losing centering.

Page layout

  • Built-in page layout templates for automatic arrangement of multiple graphs.
  • Move, resize, delete or add graphs.
  • Include data, results tables and graphs on one page.
  • Import images as .TIF, .PCX, .BMP, .JPG .GIF, .WMF (Windows) or PICT (Mac) files.
  • Create portrait and landscape pages in one file.
  • Tools to equalize the size, and align axes, of several graphs.
  • 6 levels of zoom.
  • Layouts will automatically update when you edit a graph or data plotted on the graph – even if the graph comes from a different Prism file.
  • Draw lines, arrows, boxes, circles, ovals and arcs.
  • Add text, including Greek letters and super- or subscripts.
  • Align objects (left, right, center, top, bottom) or align graphs by X or Y axes.
  • Send to back. Bring to front.
  • Duplicate objects or groups of objects.
  • Group and ungroup objects.
  • Use rulers, snap to guidelines, superimpose grid.
  • Choose any color for any data set or object, and define your own palette of custom colors.
  • Embed OLE objects, such as equations and WordArt. (Windows only)
  • Paste any portion of a data, results or info table onto a graph or layout.
  • Customize the table with a border, grid lines, or titles.
  • Create text tables as well as numerical tables.
  • Place any number of tables on one graph or layout page.
  • Prism automatically updates tables when you edit the data.
  • Print in color or black and white.
  • Copy and paste into Word or PowerPoint.
  • One-click copy and paste into a new slide in PowerPoint (Windows only).
  • Embed or link a Prism graph into PowerPoint or Word using OLE (Windows only).
  • Export as a wmf (Windows) or pict (Mac) file.
  • Export as a bitmap in png, bmp, jpg, or tif format at up to 1200 dots per inch.
  • Choose RGB or CMYK colors. Choose whether to include background color in exported file.
  • Email an entire file, or just one page, right from Prism (Windows only).
  • Post one graph or layout, or all graphs or layouts, to a web server.
Rate this page
Comment